If second messenger are still new to you (or complicated) it's worth learning more about them. How would (does) second messenger fit into NBE?........I'd start with hormone biosynthesis, which in layman's term goes something like this.
1) cell diffusion (hormones passes through the cell membrane)
2) picks up the second messager
3) second messenger moves into the nucleus
4) completion (synthesis) of DNA (activation) —> new growth..........(aka-boob growth)
Now, I've passed over several key points of synthesis, but, hopefully you get the idea.
Second messengers are:
Forskolin
Calcium
A couple new one's
Zinc (long term activation, not from cascade)
Caffeine (hot damn)
(Possibly- manganese)....think pineapple
One other note: adrenalin makes hormone receptors more sensitive, guess the body builders already know this. I think NBE stacking makes sense though, as an example, say immediately after a fitness routine, NBE supplementation should commence, Imo it would follow: (when blood flow and heart rate are peak)
Pre-workout: Take anti-androgen
Post work out: MSM, E2/Phytoestrogens, pro-aromatase, calcium, ATP (astaxanthin), a few slices of pineapple, hydration, I might be missing some, feel free to add to the post work out routine.
As listed, second messengers improve the biosynthesis of hormones, so does ATP (Adenosine triphosphate, and
Astaxanthin works quite well for this ), G protein-coupled estrogen receptors (GPER), activation of GPER comes from estrogen, which is the result of adenylyl cyclase and cAMP-dependent signaling.
Cell signaling
http://users.rcn.com/jkimball.ma.ultrane...Messengers
(15-03-2016, 12:40 AM)Lotus Wrote: Imo, second messengers are an aromatase for cell bio-synthesis. In other words, first messengers are about cell diffusion, while second messengers boost the intercellular action for DNA syntheses (or cell reaction).
Forskolin is supplement example of a second messenger. Calcium is a second messenger too, and underestimated for NBE. Btw, boosting vitamin D and calcium upregulates ovarian aromatase.
Second messengers are intracellular signaling molecules released by the cell to trigger physiological changes such as proliferation, differentiation, migration, survival, and apoptosis. Secondary messengers are therefore one of the initiating components of intracellular signal transduction cascades. Examples of second messenger molecules include cyclic AMP, cyclic GMP, inositol trisphosphate, diacylglycerol, and calcium. The cell releases second messenger molecules in response to exposure to extracellular signaling molecules—the first messengers. First messengers are extracellular factors, often hormones or neurotransmitters, such as epinephrine, growth hormone, and serotonin. Because peptide hormones and neurotransmitters typically are biochemically hydrophilic molecules, these first messengers may not physically cross the phospholipid bilayer cell membrane to initiate changes within the cell directly—unlike steroid hormones, which usually do. This functional limitation necessitates the cell to devise signal transduction mechanisms to transduce first messenger into second messengers, so that the extracellular signal may be propagated intracellularly. An important feature of the second messenger signaling system is that second messengers may be coupled downstream to multi-cyclic kinase cascades to greatly amplify the strength of the original first messenger signal.[1][2] For example, Ras.GTP signals link with the Mitogen Activated Protein Kinase (MAPK) cascade to amplify the allosteric activation of proliferative transcription factors such as Myc and CREB. (Wiki) https://en.m.wikipedia.org/wiki/Second_messenger_system
Interesting in that calcium supplementation is suggested when taking PM, I wonder if they new that calcium improves cell biosynthesis, or was it from PM lowering the parathyroid?, hmmmm.
Anyways, The daily recommended is 1000 mg to 1200 mg, although topping out at 2500 mg, certain restrictions (interactions) need to be observed though, (check with your doctor) e.g calcium channel blockers. Carbs increase the absorption of calcium while coffee decreases it (MF'er) 
Here is some excellent info on second messengers:
Second Messenger
http://users.rcn.com/jkimball.ma.ultrane...ml#IP3_DAG
Bottom line, here's one example of thinking inside the box (the cell) lol makes sense. Meaning looking at cell diffusion and how boosting the calcium signal (via calcium ion channels) improves the DNA synthesis of NBE/Hrt, just my opinion on that part though. 
Calcium
http://umm.edu/health/medical/altmed/supplement/calcium
