I see a problem with Puerarin, which is in Pueraria Mirifica (PM). In other words, aromatase is cut 50% by puerarin.
Regulation of aromatase P450 expression by puerarin in endometrial cell line RL95-2
It was demonstrated that in untreated RL95-2 cells, when c-jun was knocked down, the expression of P450arom exhibited >50% decrease at both mRNA and protein levels, which meant c-jun was essential for P450arom overexpression. Therefore, when puerarin was used, c-jun was down-regulated and so was P450arom.
http://www.jcimjournal.com/en/fulltext2.aspx?articleID=167219772008101017
Results
3.1 Effects of puerarin on P450arom expression and activity To find out whether puerarin (Figure 1A) inhibited aromatase and led to its function of endometriosis treatment, P450arom expression in RL95-2 cells treated with puerarin (10－7 mol/L) or dimethyl sulphoxide (DMSO) was estimated by quantitative RT-PCR and Western blot method. A significant decrease of P450arom expression at mRNA level by 10－7 mol/L puerarin treatment in RL95-2 compared with DMSO treatment was demonstrated by our group (Figure 1B). Furthermore, there was a time-course effect on P450arom mRNA expression at 10－7 mol/L puerarin, which was significantly reduced at 6 h, reached the bottom (>70%) at 12 h and returned to normal at 24 h (Figure 1C).
Regulation of aromatase P450 expression by puerarin in endometrial cell line RL95-2
It was demonstrated that in untreated RL95-2 cells, when c-jun was knocked down, the expression of P450arom exhibited >50% decrease at both mRNA and protein levels, which meant c-jun was essential for P450arom overexpression. Therefore, when puerarin was used, c-jun was down-regulated and so was P450arom.
http://www.jcimjournal.com/en/fulltext2.aspx?articleID=167219772008101017
Results
3.1 Effects of puerarin on P450arom expression and activity To find out whether puerarin (Figure 1A) inhibited aromatase and led to its function of endometriosis treatment, P450arom expression in RL95-2 cells treated with puerarin (10－7 mol/L) or dimethyl sulphoxide (DMSO) was estimated by quantitative RT-PCR and Western blot method. A significant decrease of P450arom expression at mRNA level by 10－7 mol/L puerarin treatment in RL95-2 compared with DMSO treatment was demonstrated by our group (Figure 1B). Furthermore, there was a time-course effect on P450arom mRNA expression at 10－7 mol/L puerarin, which was significantly reduced at 6 h, reached the bottom (>70%) at 12 h and returned to normal at 24 h (Figure 1C).
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