Hi Lotus!

Lovely as ever!! 

Continued good luck and well wishes to you! 

Hugs! 

~H

Hi DD's, thank you for saying hello, it's always good to hear from you. Best regards L. 

Hi Lotus, I hope you're doing better lately. Boobs look as fine as always.  

I just hit 41 month mark on HRT and one month on RC extract and I can tell its working, I think you hit nail to the head with this one. Another month or two to show just how good addition it is.

I wish you best of luck with health issues, there's so much to talk about and to live for.

Hi Heaven's Night, thank you.  congrats on hitting 41 months of HRT   you've worked so hard at it. I think the RC (or pio) takes about 8 months or longer to see hip/butt benefits... or maybe longer for slow metabolizers. We'll talk soon. 

The Topical Protocol is a breakthrough in the advancement of the science of feminizing breast development!

100%!!!! I started the topical and went from my tiny A's to much fuller B's in less than 6 weeks. The only change I made was adding the topical regimen. Thanks Lotus and Lara for all the info!!

Hi Brie, that's great news.   here's hoping you hit C-cup's in 4 weeks 

Lotus,

It’s great to hear from you.  You look marvelous, as always

I’m contemplating starting your topical routine but my one hesitation is that dhea could also raise T if it’s not adequately suppressed.  And I’m not sure if mine is.  I don’t want to be accidentally undermining my anti-androgens, if that’s the case.  

I’ve had good results with boosting my estrogen pathways by using Forskolin and L-Argentine with B6.  MSM screwed with my JAK pathway and led to some serious eczema-like symptoms.  

Hope to hear from you soon!  

Hi E, thank you   I think i can provide some numbers via lab results from Dr. Powers that show how using progesterone/DHEA fared for me... it'll also show my DHT using topical testosterone to prevent penis atrophy. Originally, I was planning on bottom surgery and read Dr. Powers made a T cream to help restore the atrophy before having bottom surgery, in fact it helped many (including in adult film industry people). The one thing i didn't expect was leukemia, and being that my immune system is squat I'm susceptible to any and all infections. So, no surgery for me. Be advised to all that using T creams or gels you may grow hair on the underside of your penis. So, I checked and I did grow hair there.. albeit a very small area. I mention this nugget because its not talked about so much. The other reason for use of T-cream before bottom surgery is to get you're vagina the depth you prefer... 6 inches is about the deepest and that's stretching it... pun included. 

I'll try to post the lab results asap. 

Hi Lotus, I hope you're doing better lately. Boobs look as fine as always.  

I just hit 41 month mark on HRT and one month on RC extract and I can tell its working, I think you hit nail to the head with this one. Another month or two to show just how good addition it is.

I wish you best of luck with health issues, there's so much to talk about and to live for.

Hi Heaven's Night, thank you.  congrats on hitting 41 months of HRT   you've worked so hard at it. I think the RC (or pio) takes about 8 months or longer to see hip/butt benefits... or maybe longer for slow metabolizers. We'll talk soon. 

Any time.

So you're speculating that RC has a cumulative effect? That sounds a lot like how both Pio and Volufiline seem to work, with both I have noted first month being rather slow, but consecutive months pick up the pace a bit. I wouldn't be surprised at all if all PPAR-y stuffs have this effect. We will see, so far I'm very optimistic and want to keep going at it. I haven't measured, but I notice very obvious difference from a month ago so I'm sure its already doing its thing.

Lotus,

It’s great to hear from you.  You look marvelous, as always

I’m contemplating starting your topical routine but my one hesitation is that dhea could also raise T if it’s not adequately suppressed.  And I’m not sure if mine is.  I don’t want to be accidentally undermining my anti-androgens, if that’s the case.  

I’ve had good results with boosting my estrogen pathways by using Forskolin and L-Argentine with B6.  MSM screwed with my JAK pathway and led to some serious eczema-like symptoms.  

Hope to hear from you soon!  

Hi E, thank you   I think i can provide some numbers via lab results from Dr. Powers that show how using progesterone/DHEA fared for me... it'll also show my DHT using topical testosterone to prevent penis atrophy. Originally, I was planning on bottom surgery and read Dr. Powers made a T cream to help restore the atrophy before having bottom surgery, in fact it helped many (including in adult film industry people). The one thing i didn't expect was leukemia, and being that my immune system is squat I'm susceptible to any and all infections. So, no surgery for me. Be advised to all that using T creams or gels you may grow hair on the underside of your penis. So, I checked and I did grow hair there.. albeit a very small area. I mention this nugget because its not talked about so much. The other reason for use of T-cream before bottom surgery is to get you're vagina the depth you prefer... 6 inches is about the deepest and that's stretching it... pun included. 

I'll try to post the lab results asap. 

Lotus, thank you to taking the time to respond.   

But before you started your topical routine, I assume you had already tanked your T-levels??  So if you did, a small bump in DHT would still keep you within the accepted female range?  But I’m sure I’m not in that range yet so adding it would seem sort of counterproductive?  Yes/No?  Plus, I have to get labs done to see where I’m at.  

And atrophy.  It’s a catch-22 for me.  On one hand, atrophy is a sign the anti-androgens are working. (Plus, smaller is easy to tuck away.). But on the other hand, I still use it and need a functional one.  Grrrrrr  If I had only been born differently….

Hi Lotus, I hope you're doing better lately. Boobs look as fine as always.  

Hi Lara, thank you for the kind words  lol, things (my health) got worse, my CML (chronic myeloid leukemia) took a turn for the worse, a recent blood work test showed the CML went from 93% cells tested positive for cancer in August to 100% cells test were cancer. So it's like everything is next level pain and fatigue. No exaggeration I need to sleep every two hours. But, my boobs are (and have been) the healthiest part of my being   btw you look great. 

So you're speculating that RC has a cumulative effect? That sounds a lot like how both Pio and Volufiline seem to work, with both I have noted first month being rather slow, but consecutive months pick up the pace a bit. I wouldn't be surprised at all if all PPAR-y stuffs have this effect. We will see, so far I'm very optimistic and want to keep going at it. I
haven't measured, but I notice very obvious difference from a month ago so I'm sure its already doing its thing.

Cumulative for body feminization yes, imho it has immediate impact when used on areolas and nipples. I find further evidence of benefiting the latter when used with E2 and BO. In a past post I shared scientific evidence of bovine ovaries being tested with E2 (estradiol) injection. The showed how E2/BO has much more DNA/RNA synthesis than if used separately. Now, taking the above scientific literature and extrapolate the information I concluded an experiment was needed to see if BO and E2 act synergisticially... and "yup", it sure the hell works. 

So many things to discuss, here's a couple below. I wish I was healthy enough to discuss some new theories i have, and enhance things I've already shared that need further follow up. Thanks for helping others understand the TCP (Topical Cream Protocol). 

Estradiol enhances the stimulatory effect of insulin-like growth factor-I (IGF-I) on mammary development and growth hormone-induced IGF-I messenger ribonucleic acid
https://pubmed.ncbi.nlm.nih.gov/7867584/

"E2 significantly enhanced the effects of IGF-I on mammary development. Plus, E2 treatment improves collagen synthesis by making new collagen" from the study.

A β-hydroxybutyrate shunt pathway generates anti-obesity ketone metabolites
https://pubmed.ncbi.nlm.nih.gov/39536746/

Oh btw, my mind works  . Turned out i was struggling with cluster headache/migraine  .. ta-da!
But, lol on the other hand I forget to acknowledge a few people in my last group posting. Thank you wee2er and Brianna and others I need to follow up in messages, please forgive. Like I said before and it's worth repeating... if I'm not posting it's because of battling illness.   I do plan on adding more content to the TCP thread. 
 

Attached Files

---

Here's the information on E2 / BO I shared sometime ago. I have also copy of the PDF. 

The interaction of estradiol-17b with mature bovine endometrial tissue, and with isolated nuclei has been studied. The hormone binds to an insoluble nuclear fraction. This fraction contains membranes and evidence is presented to show that estradiol is bound to the nuclear membrane. Incubation of isolated nuclei and microsome fractions with estradial-17b shows that the hormone binds essentially instantaneously to microsomes and nuclei. Such binding is nonsaturable up to estradiol-17b concentrations as great as 2.5 x 10m6 moles per mg of membrane protein and it is likely that this interaction is not biologically significant. A second form of binding is observed in nuclei which is of higher affinity and saturable, with 507 •t 47 sites per nucleus. This class of binding sites is found to be blocked in cattle that are maintained in artificially induced estrus by feeding with diethylstilbesterol. The high affinity binding site is present only in the mature endometrium and is absent in nuclei from the mature myometrium (the muscular tissue surrounding the endometrium in the uterus) and the immature uterus. Potent estrogenic agents compete effectively with estradiol-17b for binding to this site, whereas weak estrogenic steroids (such as progesterone and testosterone) are inefficient competitors. The sensitivity of the high affinity site to pH and hydrolytic enzymes has been studied and compared with the effect of such agents on the low affinity, nonspecific, membrane site. Such a comparison underscores the inherent differences between these two sites of interaction. The thermodynamic parameters of the high affinity binding site have been measured and compared to reported values for the specific cytoplasmic receptor binding site. The nuclear high affinity binding site possesses a favorable free energy of interaction as a result of entropic forces (AS0 = +36.4 cal deg-l mole-‘, AH0 = - 0.66 Cal mole-‘). The number of high affinity nuclear sites varies dramatically during the estrous cycle in a bicyclic fashion. Sites are available during estrus and diestrus, whereas they are blocked in later metestrus and proestrus. * This work was supported by United States Public Health Service Grant CA-10871 and by the American Cancer (Iowa Division) Grant P451. $ Research Career Development Awardee of the National tutes of Health, Grant GM-48410. Society InstiEstradiol-17b causes cell proliferation in endometrial tissue approximately 48 hours after blood levels of the steroid rise during the estrous cycle (1). Prior to cell division there is an initial increase in ribosomal RNA synthesis (2) and 24 hours later an increase in DNA synthesis (3). It has been proposed that the hormone itself mediates at least part of this response by interacting directly with the genome (4-6). Following injection of the hormone in the rat, or direct incubation of the uterus with the hormone, a portion of the estradiol becomes associated with the uterine nuclear fraction in a specific manner (7-9). Although the nuclear fraction has not been analyzed in great detail, some evidence has been presented that estradiol binds to the chromatin fraction (7). We will present data which support the proposal that in vivo, estradiol binds to the nuclear membrane, which appears to be an integral part of chromatin as isolated. During in vitro incubation using isolated nuclei, the hormone binds nuclei both at a nonsaturable low affinity site (in a rapid reaction, which resembles its binding to isolated microsomal material) and also at a saturable high affinity site (with a measurable rate) which we propose is the same site as that involved in incubations with intact tissue. We also report on the thermodynamic parameters of hormone binding and on the sensitivity of the high affinity site to various hydrolytic enzymes and to pH. In several of these properties, the high affinity sites on the nucleus are clearly different from the cytoplasmic receptors and we will argue that the high affinity nuclear binding site which is present in endometrial tissue is a discrete entity in itself and is distinct and separate from the cytoplasmic receptor site. Finally, we have found that the availability of the nuclear high affinity sites varies during the estrous cycle, though this variation is not reflected in the concentration of cytoplasmic receptors in the cell, which remains relatively constant. MATERIALS 

Finally, we note that the in vitro high affinity site is absent in immature calves and also in mature heifers during the late metestrus and the proestrus stages of the estrous cycle. During diestrus the blood level of estradiol is low (42) and the tissue is prepared to respond, and thus the availability of open sites 011 the nucleus seems reasonable. During proestrus the blood level of estradiol is rising rapidly, the tissue is visibly responding and presumably the sites are unavailable because they are blocked by the bound endogenous hormone. As expected the number of sites increases through estrus where the hormone has completed its stimulation of endometrial proliferation, and the blood level of estrogen is declining. However, during early metestrus and late metestrus when the blood level of estradiol is quite low, the number of nuclear sites become vanishingly small, even though it is unlikely that at this stage of the cycle that the sites would be blocked by endogenous hormone. However, it is known that animals in late metestrus cannot reinitiate an appro21. 

The Binding of Estradiol-17β to the Bovine Endometrial Nuclear Membrane
https://www.sciencedirect.com/science/article/pii/S0021925819429256

Tomorrow, I'll be talking about how adding Olive Oil (taken orally, 2-3 tablespoons) within 4 hours of taking E2, DHEA or progesterone enhances the benefits of breast growth. 

Hi Lotus, I've missed you. I'm so sorry about the damn cancer battle, its so unfair that you have to deal with it.

I will gladly help, you can't be here explaining everything all the time, but I'm online pretty much daily and people keep asking about it. Seems like everybody who has tried the topicals so far are getting positive results, its truly a ground breaking discovery.

I copied and saved all that text about the BO E2 connection, I'm trying to build up all this information into a text file and get every study saved I can. I should go on a digging spree on BN and save everything I can think of being useful. Somehow I have a feeling that effort isn't wasted.

I've made interesting observation during last five months, the cayenne extract with BO appears to be working well, there's a visible difference in pictures correlating directly with this and some other program changes. It seems that the strong alcohol does make it absorb and it works well. The study about E2 and Bovine ovary just makes this more interesting. I will be posting in more detail about this in about two weeks, there's quite a lot to talk about, and I got to update my timeline to show what's going on. 

I'm quite sure I'll get to prove what RC does. I just ordered another batch of it. The thing about olive oil, someone spilled the beans about it and I went already all in to try it as olive oil in general is super healthly, I can't wait to hear the science of it. I'm on it nineteen days in today, can't tell yet what the effect will be, but I'm confident this one's gonna hit the spot too.