Up to two months ago, I was using 2 mg of transdermal estradiol and my blood test results was estradiol 299, so I decided decrease dosage to 1.5 mg of estradiol, during 2 months, and I did test again, and now my test result is estradiol 445. I don't know what is whrong, I heard that if my blood test is made during six hours after take transdermal estradiol, the levels can be very high, not meaning that it is the real value. So I am very confused. My next medician meeting is in january, so I can't stay for so long time without know what to do... Can someone help me to understand what is wrong, please?

Something has messed up your blood test results, there's no way that is your stable E2 level on such low dosage. I'm really not sure how it works on transdermal but it absorbs fast, so I presume it can potentially make a big level spike for a short period of time after application. With injection, blood test is done just before next injection so its taken at the lowest point of cycle which gives the result of stable level that always is there. I think same idea applies with transdermal, but the interval is much shorter as it absords really quickly.

Maybe not apply any estrogel on the day of blood test before the test, only do it after? 

The numbers you're talking about are pg/ml, correct? Always mention which measurement is used so the numbers make sense. I always convert, Finnish labs use nmol/l and I think pg/ml is nicer to read.